This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry GP protein of Marburgvirus - Angola (GenBank: KU978782.1).

Infection of cells with this pseudotyped virus carrying luciferase reporter results in high level luciferase activity. See the titration result showing the undiluted pseudovirus generates signal ~100000-fold higher than uninfected control (cell alone as background). The neutralizing activity of one published mAb called MR191 was tested by using this pseudotyped virus.

As pseudovirus infectivity of rVSV without its original G is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices. Applications Anti- Marburgvirus neutralizing antibody screening at high throughput, Anti- Marburgvirus drug screening at high throughput and Marburgvirus pseudovirus transduction of target cells for viral entry and functional studies