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Specifications
- Description:P-Glycoprotein / MDR1 Drug Interaction Assay Kit
- Cat. No.:103881-814
- Format:2× 48 assays in 96-well format
- Species reactivity:Human
Specifications
About this item
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from INDIGO’s Human P-Glycoprotein/MDR1 Drug Interaction Assay Kit, All-inclusive Cell-Based Luciferase Reporter Assay Kit, 2×48 format.
- Rapidly assess drug candidates as either inhibitors, substrates, or non-substrates of P-glycoprotein
- Kit includes kit contains two aliquots of HCT-Pgp cells, Cell Recovery Medium (CRM-p), Compound Screening Medium supplemented with daunorubicin (CSM+DR), Wash Buffer, two aliquots of Lysis Reagent, reference inhibitor Tariquidar, cell culture-ready assay plate, and detailed protocol
- Exceptional cell viability post-thaw
- Consistent results lot to lot (product quality data available upon request)
The primary application of INDIGO Biosciences MDR1 drug interaction assay kit is to rapidly assess drug candidates as either inhibitors, substrates, or non-substrates of P-glycoprotein. This is accomplished by co-treating the cells with varying concentrations of the test drug and a fixed concentration of daunorubicin, a well-characterized fluorescent substrate for P-glycoprotein mediated transport. HCT-Pgp cells are a proprietary cell line that have been extensively selected for high-level expression of native P-glycoprotein. The kit contains two aliquots of HCT-Pgp cells and two black, sterile, collagen-coated 96-well assay plates. In addition to HCT-Pgp cells and assay plates, this kit includes Cell Recovery Medium (CRM-p) used to perform a rapid thaw of the –80 °C HCT-Pgp cells, Compound Screening Medium supplemented with daunorubicin (CSM+DR) used to prepare drug treatment media, Wash Buffer, two aliquots of Lysis Reagent, and the potent reference inhibitor Tariquidar.