Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Rat Farnesoid X Receptor reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against rFXR (NR1H4).

• Includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor
• Kit includes reporter cells, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed protocol
• Exceptional cell viability post-thaw
• Consistent results lot to lot (product quality data available upon request)

This is an all-inclusive cell-based luciferase reporter assay kit targeting the Rat Farnesoid X Receptor. INDIGO's rFXR reporter assay utilizes proprietary mammalian cells that have been engineered to provide constitutive expression of the rFXR. In addition to rFXR Reporter Cells, this kit provides two optimized media for use during cell culture and in diluting the user's test samples, a reference agonist, Luciferase Detection Reagent, and a cell culture-ready assay plate. The principal application of this assay is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against rFXR. This kit provides researchers with: Clear, reproducible results, exceptional cell viability post-thaw, consistent results lot to lot.