This kit is developed for qualitative detection or titer measurement of Anti-SARS-CoV-2 (B.1.351) neutralizing antibody (Spike Trimer) in human serum.

Multiple variants of SARS-CoV-2 are circulating globally and posting new challenges to human health. As concerns over the potential impacts of the variants grow, people ask if one possesses protective neutralizing antibodies (NAbs) against the variants after receiving the currently available vaccines; and if the NAb therapeutic agents are equally effective to treat the mutant infection as the wild type. To facilitate the mutant-related research, drug trials and vaccine assessments, a high-throughput assay to measure neutralizing antibodies against the mutants is in urgent need.

Your experiment will include five simple steps:
a) All reagents were returned to room temperature (20 to 25 °C) before use.
b) Make series the tested sample and control with ditlution buffer, HRP-SARS-CoV-2 Spike protein diluted with dilution buffer.
c) Add the diluted sample, Control and the HRP-SARS-CoV-2 Spike protein add the plate respectively.
d) Wash the plate and add TMB or other colorimetric HRP substrate.
e) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.