The NEBNext Ultra II FS DNA Library Prep Kit for Illumina provides a fragmentation system: a fast and reliable solution for DNA fragmentation and library construction. A new DNA fragmentation reagent is combined with end repair and dA-tailing reagents, allowing these steps to be performed in the same tube, with no clean-ups or sample loss. The same fragmentation protocol is used for any input amount (100 pg–500 ng), or GC content.

• Perform fragmentation, end repair and dA-tailing with a single enzyme mix
• Experience reliable fragmentation with a single protocol, regardless of input amount or GC content
• Prepare high quality libraries from a wide range of input amounts: 100 pg-500 ng
• Generate high yields with increased reaction efficiencies and minimized sample loss
• Includes SPRIselect beads

The kit also includes the NEBNext Ultra II Ligation Master Mix for adaptor ligation, the NEBNext Ultra II Q5 Master Mix for uniform, high-fidelity library amplification, and the gold standard SPRIselect size selection and clean-up beads. Please note that adaptors and primers are not included in the kit and are available separately.