Our primary human cells offer unmatched consistency and a more biologically relevant tool than other cell types. Our cells are antibody-free and come from a deep inventory to help researchers with reproducibility and improved human insights.

• Highly proliferative and healthy (~85% viability)
• Antibody free and more biologically relevant
• Offered with high cell count (~1 million cells per vial)
• Well-cited in scientific journals
• Ideal for 2D and 3D cultures, microfluidic devices and co-cultures

Primary Human Lung Fibroblast Cells (ACBRI469) were isolated by elutriation from normal human lung tissue without the use of antibody labels.
Each vial will initiate a 25 to 75 cm sq cell culture. These primary cells are isolated, frozen and qualified using Cell Systems Certified Media and Reagents--which are specified for use in the research environment.
Cell Systems primary cells are exclusively from normal, site-specific human tissue specimen, and available in frozen vial or proliferating flask.

These cells were originated using Cell Systems Complete Serum-Free Medium (SF-4Z0-500), and subsequently grown and passaged in Cell Systems Complete Medium (4Z0-500). They are available at Passage 3 [<12 cumulative population doublings] cryopreserved in Cell Systems Cell Freezing Medium™ (4Z0-705). This vial will initiate a Passage 4 cell culture in a 75 cm² flask. Each vial contains more than 1×10⁶ cells.

Each vial of cells is shipped with vials of Bac-Off® (antibiotic) and CultureBoost™ (animal derived growth factors) at no additional cost.

Standard Tests:
HIV Serologic Test (donor level HIV AB EIA) - Negative
HIV PCR Test (frozen cell pool by CLIA Licensed Clinical Lab) - Negative
Hepatitis B (HBV) PCR Test (frozen cells) - Negative
Hepatitis C (HCV) PCR Test (frozen cells) - Negative
Mycoplasma spp. (frozen cells) (ATCC method by CLIA Licensed Clinical Lab) - Negative

Vimentin: >91% positive by immunofluorescence