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Specifications
- Description:pMAL-c6T vector
- Size:10 μg (50 μl)
- Cat. No.:76423-414
Specifications
About this item
The pMAL-c6T Vector is designed to produce maltose-binding protein (MBP) fusions in the cytoplasm.
- pMAL-c6T is the recommended vector for use with the NEBExpress MBP fusion and purification system
- Fusion to MBP significantly enhances the solubility and proper folding of proteins in E. coli
- Includes TEV Protease site, allowing protein to be cleaved from MBP using TEV Protease (NEB #P8112)
- Two-step purification: amylose elution followed by TEV Protease cleavage and Ni resin isolation
The MBP has been engineered for tighter binding to amylose resin. The vector expresses an N-terminal his-tagged mal E gene followed by a multiple cloning site containing a TEV protease recognition sequence, allowing MBP to be cleaved from the protein of interest after purification.