GFAP is a 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas.

Applications: This antibody is recommended for use in immunohistochemistry (1:500 - 1:1,000) and western blotting (1:1,000 - 1:2,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

Type: Primary
Antigen: GFAP
Clonality: Monoclonal
Clone: 2A5
Conjugation: Unconjugated
Epitope: The epitope for this antibody has been mapped to the N-terminal region of the alpha-helical coiled-coil rod-region of human GFAP isotype I, specifically within amino acids 71-217.
Host: Mouse
Isotype: IgG1
Reactivity: Pig, human, mouse, rat, cow