Thermostable RNase H is an endoribonuclease that is functional at high temperatures and selectively hydrolyzes the phosphodiester bonds of an RNA strand in an RNA:DNA hybrid molecule, while leaving the DNA strand intact.

• Recombinant enzyme supplied with 10X Reaction Buffer
• Does not digest single or double-stranded DNA
• Active and stable above 37 °C over a broad temperature range
• Requires magnesium chloride for activityInactivated with proteinase K treatment or addition of excess EDTA

In addition, Thermostable RNase H does not degrade single- or double-stranded RNA or DNA. While the Thermostable and E. coli RNase H homologs both exhibit these same endoribonucleolytic properties, the thermostable enzyme displays activity at higher temperatures with optimal activity above 65 °C. These properties allow use in applications where higher assay temperatures are desired.