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Specifications
- Application:
- One step:Yes
- Description:Luna® Cell Ready One-Step RT-qPCR kit
- Size:100 reactions
- Environmentally Preferable:
- Cat. No.:76407-356
Specifications
About this item
The Luna® Cell Ready One-Step RT-qPCR Kit provides all the necessary components for direct, dye-based RNA detection and quantitation, bypassing the need for RNA extraction and purification.
- Contains reagents for 100 lysis preps and 500 one-step RT-qPCR reactions (dye)
- Effective cell lysis preparation from 10 to 100000 cells across numerous cell lines
- Coordinated cell lysis, RNA release, and genomic DNA removal in a fast 15-minute protocol
- Luna WarmStart RT paired with Hot Start Taq for increased thermostability and room temperature setup
Coordinating the actions of DNase I and the Luna Cell Ready Protease, the Luna Cell Ready Lysis Module offers a simple alternative workflow resulting in effective cell lysis, RNA release, and genomic DNA removal simultaneously in a 15-minute protocol. The Lysis Module includes a unique Luna Cell Ready RNA Protection Reagent that maintains RNA integrity during cell lysis. The lysis capacity spans 10 to 100000 cells in a 50 µl lysis reaction. Up to 2 µl of lysate (equivalent to RNA from 0.2 to 4000 cells) can be transferred into 20 µl downstream RT-qPCR reactions.
1) The Luna Cell Ready Lysis Module
2) The Luna Universal One-Step RT-qPCR Kit.