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Specifications
- Application:RT-qPCR probe
- One step:Yes
- Description:Luna® Probe One-Step RT-qPCR kit (No ROX)
- Size:2500 reactions
- Environmentally Preferable:
- Cat. No.:76423-404
Specifications
About this item
In the Luna Probe One-Step RT-qPCR Kit (No ROX), Hot Start Taq DNA Polymerase is combined with a novel WarmStart-activated reverse transcriptase, allowing dual control of enzyme activity via reversible, aptamer-based inhibition.
- For rapid, sensitive, and precise probe-based qPCR detection and quantitation of RNA targets
- For instruments that do not require the ROX passive reference dye
- Convenient master mix formats and user-friendly protocols simplify reaction setup
- Non-interfering, visible tracking dye helps to eliminate pipetting errors
- WarmStart RT paired with Hot Start Taq increases reaction specificity and robustness
This temperature-dependent activation helps to prevent undesirable non-specific priming and extension prior to thermocycling, providing added security for setting up reactions at room temperature. The engineered WarmStart Luna Reverse Transcriptase also possesses higher thermostability than many other RTs, allowing an optimal reaction temperature of 55 °C. For difficult targets/templates, higher RT step temperatures of up to 60 °C can be used without compromising Luna performance. Note that to ensure full activation of the WarmStart Luna RT, incubation at temperatures lower than 50 °C is not recommended. This formulation contains no reference dye and is compatible with any instrument that does not require ROX (if ROX normalization is needed, ROX can be added.)