The Luna SARS-CoV-2 RT-qPCR Multiplex assay kit is optimized for real-time qualitative detection of SARS-CoV-2 nucleic acid using hydrolysis probes.

• Optimized primer/probe mix with different fluorophores supports multiplexing, increased throughput
• Internal control RNase P gene reverse primer modified to reduce background ampliflication
• Luna WarmStart RT and Hot Start Taq for increased reaction specificity and room temperature setup
• Positive control template (SARS-CoV-2 N-gene cloned into a plasmid) provided
• Luna 4X mix contains a non-interfering, visible blue tracking dye to eliminate pipetting errors

It features the Luna Probe One-Step RT-qPCR 4X Mix with UDG, an optimized SARS-CoV-2 Primer/Probe mix containing primers and probes specific to two regions of the SARS-CoV-2 virus N-gene, and a positive control template. The probes have been modified to contain different fluorophores (N1, HEX; N2, FAM) to enable simultaneous observation on two different channels of a real-time instrument. To ensure the integrity of the input material and absence of inhibition, an internal control (IC) primer and probe set, designed to amplify the human RNaseP gene, is also provided in the primer mix. The reverse primer of this target has been modified from the CDC design to target an exon/exon boundary to reduce background amplification from possible contaminating genomic DNA. Amplification of the IC is observed in the Cy5 channel.