About this item
A reverse transcriptase engineered to deliver phenomenal results.
- Quantification of gene expression
- RT-qPCR
- Next generation sequencing
- Low RNA concentration
- Difficult templates
- Longer cDNAs in less time
How would you construct the ideal reverse transcriptase? It would have to make very long cDNAs at high yields. It would have to be able to work from very small amounts of input RNA. And of course, it would have to work fast. FastGene Scriptase II checks all three boxes, and it does it at a reasonable price. Here’s how. First, mutations are inserted into the RNase H domain of native MuLV‘s reverse transcriptase delivering a higher yield of full-length cDNAs. Next, changes are made to increase the enzymes natural thermal stability providing a more robust enzyme that doesn’t lose processing power, even at elevated temperatures. Finally, the reaction buffer composition is optimized for high speed production of cDNAs from incredibly low levels of input RNA. This unique combination of size, speed, and production makes FastGene Scriptase II the optimal choice for even the most complex applications requiring cDNAs, such as RT-qPCR and NGS.
FastGene Scriptase II has a modified RNase H domain which reduces the active lysis of the template RNA. By slowing this degradation, RT enzyme allows the template to remain intact for just long enough to deliver cDNAs of greater length. It is common to achieve cDNA sizes of up to 12 kBp. The quality of this engineered enzyme is so exceptional that typical incubation times of 50 minutes can be reduced to as little as five.
FastGene Scriptase II comes in three convenient formats. You can purchase the Scriptase II reverse transcriptase with just the bare essentials: 5x RT buffer, dNTPs, DTT, and some RNAse-free water (LS53). You can purchase the complete cDNA synthesis (LS63), which contains everything in the LS53 format plus everything else you’ll need to optimize the perfect cDNA synthesis blend for your particular application: random hexamer primers, oligo dT primers, and RNase inhibitor. The last option is the ReadyMix format (LS64 and LS65), for those labs that need results quickly and without the need to optimize.