Specifications

• Conjugation:
  Unconjugated
• Protein/Peptide Type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Paguma larvata
• Tag sequence:
  His Tag (SPR verified)
• Storage Conditions:
  –70 °C
• Endotoxin Content:
  Less than 1.0 EU per μg by the LAL method.
• Biological Activity:
  Paguma larvata ACE2, His Tag (Cat. No. AC2-P5248) immobilized on CM5 Chip can bind SARS S protein RBD, His Tag (Cat. No. SPD-S52H6) with an affinity constant of 0.101 μM as determined in a SPR assay (Biacore T200) (QC tested). Paguma larvata ACE2, His Tag (Cat. No. AC2-P5248) immobilized on CM5 Chip can bind SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) with an affinity constant of 29 μM as determined in a SPR assay (Biacore T200) (Routinely tested). Paguma larvata ACE2, His Tag (Cat. No. AC2-P5248) immobilized on CM5 Chip can bind SARS-CoV-2 S1 protein, His Tag (Cat. No. S1N-C52H4) with an affinity constant of 47.5 μM as determined in a SPR assay (Biacore T200) (Routinely tested). Paguma larvata ACE2, His Tag (Cat. No. AC2-P5248) immobilized on CM5 Chip can bind SARS S1 protein, His Tag (Cat. No. S1N-S52H5) with an affinity constant of 0.796 μM as determined in a SPR assay (Biacore T200) (Routinely tested). Loaded SARS-CoV-2 S protein RBD, Fc Tag (Cat. No. SPD-C5255) on Protein A Biosensor, can bind Paguma larvata ACE2, His Tag (Cat. No. AC2-P5248) with an affinity constant of 1.17 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• Gene ID:
  Q56NL1-1
• Protein Synonyms:
  ACEH|ACE-2|ACE2
• Protein/Peptide Name:
  ACE2
• Purity:
  >90% as determined by SDS-PAGE.
• Molecular Weight:
  85.7 kDa
• Endotoxin Level:
  Low
• Formulation:
  Delivered as bulk protein in a 0.2 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH7.5 with glycerol as protectant.
• Shipping Temperature:
  Dry Ice