Mouse Recombinant EGF (from HEK293 Cells)

About this item

Mouse EGF Protein, Fc Tag, ACROBiosystems

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Specifications

Conjugation:
Unconjugated
Protein/Peptide Type:
Recombinant
Species:
Mouse
Tag sequence:
Fc Tag
Storage Conditions:
–20 °C
Endotoxin Content:
Less than 1.0 EU per μg by the LAL method.
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized Mouse EGF R, His Tag (Cat. No. EGR-M5224) at 5 μg/mL (100 μL/well) can bind Mouse EGF, Fc Tag (Cat. No. EGF-M5265) with a linear range of 0.2-5 ng/mL (QC tested). Captured Mouse EGF R, His Tag (Cat. No. EGR-M5224) on CM5 Chip via anti-His antibody can bind Mouse EGF, Fc Tag (Cat. No. EGF-M5265) with an affinity constant of 5.44 nM as determined in SPR assay (Biacore T200 ) (Routinely tested).
Gene ID:
P01132-1
Protein Synonyms:
EGF|URG|HOMG4
Protein/Peptide Name:
EGF
Purity:
>95% as determined by SDS-PAGE.
Molecular Weight:
32.5 kDa
Endotoxin Level:
Low
Formulation:
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Shipping Temperature:
RT