Specifications

• Conjugation:
  Unconjugated
• Protein/Peptide Type:
  Recombinant
• Species:
  Human
• Tag sequence:
  His Tag&Tag Free (MALS verified)
• Storage Conditions:
  –20 °C
• Endotoxin Content:
  Less than 1.0 EU per μg by the LAL method.
• Biological Activity:
  Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free (Cat. No. CDD-H52W1) captured on CM5 Chip via anti-His antibody can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) (Cat. No. CDE-M120a) with an affinity constant of 40 nM as determined in SPR assay (Biacore T200) (Routinely tested). Bispecific T-cell Engager (CD3 X BCMA) immobilized on CM5 Chip can bind Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free (Cat. No. CDD-H52W1) with an affinity constant of 31.8 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
• Gene ID:
  P07766-1 (CD3E) & P04234-1 (CD3D)
• Protein Synonyms:
  CD3 delta / epsilon|CD3 delta & CD3 epsilon|CD3E & CD3D
• Protein/Peptide Name:
  CD3E & CD3D
• Purity:
  >90% as determined by SDS-PAGE.
• Molecular Weight:
  16.6 kDa (CD3E) and 13.4 kDa (CD3D)
• Endotoxin Level:
  Low
• Formulation:
  Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.
• Shipping Temperature:
  RT