Human Recombinant CD3E & CD3D (from HEK293 Cells)

Human Recombinant CD3E & CD3D (from HEK293 Cells)

Human CD3 epsilon & CD3 delta Heterodimer Protein, His Tag&Tag Free (MALS verified), ACROBiosystems

Conjugation:
Unconjugated
Protein/Peptide Type:
Recombinant
Species:
Human
Tag sequence:
His Tag&Tag Free (MALS verified)
Storage Conditions:
–20 °C
Endotoxin Content:
Less than 1.0 EU per μg by the LAL method.
Biological Activity:
Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free (Cat. No. CDD-H52W1) captured on CM5 Chip via anti-His antibody can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) (Cat. No. CDE-M120a) with an affinity constant of 40 nM as determined in SPR assay (Biacore T200) (Routinely tested). Bispecific T-cell Engager (CD3 X BCMA) immobilized on CM5 Chip can bind Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free (Cat. No. CDD-H52W1) with an affinity constant of 31.8 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Gene ID:
P07766-1 (CD3E) & P04234-1 (CD3D)
Protein Synonyms:
CD3 delta / epsilon|CD3 delta & CD3 epsilon|CD3E & CD3D
Protein/Peptide Name:
CD3E & CD3D
Purity:
>90% as determined by SDS-PAGE.
Molecular Weight:
16.6 kDa (CD3E) and 13.4 kDa (CD3D)
Endotoxin Level:
Low
Formulation:
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.
Shipping Temperature:
RT