Specifications

• Conjugation:
  Unconjugated
• Protein/Peptide Type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Tag sequence:
  His Tag (active trimer) (MALS verified)
• Storage Conditions:
  –20 °C
• Endotoxin Content:
  Less than 1.0 EU per μg by the LAL method.
• Biological Activity:
  Measured by its binding ability in a functional ELISA. Immobilized Human HVEM, Fc Tag (Cat. No. HVM-H5258) at 5 μg/mL (100 μL/well) can bind Human LIGHT, His Tag (active trimer) (MALS verified) (Cat. No. LIT-H5242) with a linear range of 16-63 ng/mL (QC tested). Measured by its binding ability in a functional ELISA. Immobilized Human DcR3, Fc Tag (Cat. No. TNB-H5255) at 5 μg/mL (100 μL/well) can bind Human LIGHT, His Tag (active trimer) (MALS verified) (Cat. No. LIT-H5242) with a linear range of 8-63 ng/mL (Routinely tested). Human LIGHT, His Tag (active trimer) (MALS verified) (Cat. No. LIT-H5242) induced cytotoxicity in HT-29 cells. The ED50 for this effect is 1.11-3.87 ng/mL (Routinely tested).
• Gene ID:
  O43557-1
• Protein Synonyms:
  HVEM-L|CD258|LTg|TR2|LIGHT|HVEML|CD258 antigen|TNFSF14
• Protein/Peptide Name:
  Light
• Purity:
  >90% as determined by SDS-PAGE.
• Molecular Weight:
  58.3 kDa
• Endotoxin Level:
  Low
• Formulation:
  Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Shipping Temperature:
  RT