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Human Recombinant CD73 (from HEK293 Cells)
Human Recombinant CD73 (from HEK293 Cells)
Supplier:  ACROBIOSYSTEMS INC MS
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Human Recombinant CD73 (from HEK293 Cells)
Supplier:  ACROBIOSYSTEMS INC MS
Restricted Products: To process your orders without delay, please provide the required business documentation to purchase this product.

To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:

  • • State issued document with your organization's Federal Tax ID Number
  • • State issued document with your organization's Resale Tax ID Number
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  • • State Department of Health Services License
  • • Any other ID issued by the State that includes the business name & address

* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.

Specifications

  • Conjugation:
    Unconjugated
  • Protein/Peptide Type:
    Recombinant
  • Species:
    Human
  • Tag sequence:
    His Tag (HPLC-verified) (active enzyme)
  • Storage Conditions:
    –20 °C
  • Endotoxin Content:
    Less than 1.0 EU per μg by the LAL method.
  • Biological Activity:
    Measured by its binding ability in a functional ELISA. Immobilized Human CD73, His Tag (HPLC-verified) (Cat. No. CD3-H52H7) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD73 Antibody, Human IgG1 with a linear range of 0.15-5 ng/mL (Routinely tested). Anti-CD73 antibody (Human IgG1) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human CD73, His Tag (HPLC-verified) (Cat. No. CD3-H52H7) with an affinity constant of 0.164 nM as determined in a SPR assay (Biacore 8K) (Routinely tested). Loaded Anti-CD73 MAb (Human IgG1) on AHC Biosensor, can bind Human CD73, His Tag (HPLC-verified) (Cat. No. CD3-H52H7) with an affinity constant of 0.179 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
  • Gene ID:
    P21589-1
  • Protein Synonyms:
    5'-Nucleotidase|5'-NT|NTE|NT5|NT5E|CD73
  • Protein/Peptide Name:
    CD73
  • Purity:
    >90% as determined by SDS-PAGE.
  • Molecular Weight:
    59.9 kDa
  • Endotoxin Level:
    Low
  • Formulation:
    Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 120 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
  • Shipping Temperature:
    RT

Specifications