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Specifications
- Description:NEPA porator electroporation system
- Environmentally Preferable:
- Voltage Range:100 - 3000 V (1 V resolution)
- Pulse Interval:0 - 5.0 ms pulse times
- Cat. No.:103778-494
Specifications
About this item
Ohm-Checkable for consistent results.
- Impedance checks for better results
- Simple functionality without arcing
- Consistently better levels of transformation efficiency with E. coli
- Save up to 99 programs
Electroporation is a process by which one or more electrical pulses are delivered to a biological sample. These pulses result in the temporary disruption of the cell membrane, and under some conditions the cell wall. The 'porated' cells are then able to quickly take up DNA, RNA, or proteins in the surrounding buffer. The NEPA Porator is designed to deliver one or two DC exponential decay waves with constant voltage. What makes the NEPA Porator so special is its ability to first check the resistance of the sample via a trickle charge. This quick check ensures your samples' buffering conditions are in the right range, so you know that you’re applying the correct amount of energy each and every time. In many cases, a quick adjustment to the sample volume in the cuvette can bring resistance back into the optimal range so that you can proceed with your experiment. In other cases, the system helps to identify 'dirty' cells or DNA preps, so that these components can be re-purified and electroporation can move forward with the promise of consistent results.
In bacterial electroporation, trace amounts of salts often drive down resistance and result in the scary popping noise referred to as 'sparking' or 'arcing'. This can also happen when bacterial cells are over-porated, causing them to release their intracellular contents; the cascading drop in resistance that follows can ruin whole experiments. The NEPA Porator helps eliminate arcing by allowing you to 'see' a drop in resistance before electroporation even begins.
The NEPA Porator's unique ability to pre-measure the electrical impedance of samples before pulsing makes it suitable for a wide range of species. It has the capacity to pulse from 100 V all the way up to 3000 V. The lower range (100 to 300 V) is perfect for the efficient transfection of many common lines of cultured cells. The higher range (1000 V+) is ideal for organisms with a cell wall, such as bacteria, yeast, and other fungi. The NEPA Porator is used widely throughout Japan, and the data from these labs demonstrate that this system delivers exceptionally consistent results.