DNase I
Purified DNase I tested to be free of contaminating activity for the degradation of nucleic acids.
- RNase Free
- Contains a mixture of four isoenzymes from bovine pancreas (10 units/µL)
DNase I, RNase Free catalyzes the degradation of double-stranded DNA into oligonucleotides 1,2 and mononucleotides. This enzyme has been isolated as a mixture of four isoenzymes from bovine pancreas that cut preferentially next to pyrimidine nucleotides.
Reaction Conditions: 40 mM Tris-HCl (pH 7.5), 6 mM MgCl2, 2 mM CaCl2, 1 µg DNA and 1 unit enzyme in a 25 µL volume. Incubate 30 minutes at 37 °C.
: For research use only. Not for use in diagnostic procedures.
- Form:Premixed
- Enzyme Name:DNase I
- Formulation:10 mM Tris-HCl (pH 7.5), 10 mM CaCl2, 10 mM MgCl2 and 50 % glycerol (v/v). (Dilutions should be made in this buffer.)