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- Size:600 U
- Storage Conditions:–30...–10 °C
- Enzyme Name:AMV Reverse Transcriptase
- Enzyme Synonyms:RT
- Concentration:20 - 25 units/µl
- Tested Applications:Sequencing, Mutagenesis, cDNA Synthesis, cDNA Cloning, Primer Extension
- Cat. No.:PAM9004
- Supplier no.:M9004
AMV RT catalyzes DNA polymerization using template DNA, RNA or RNA:DNA hybrids.
- For sequencing, mutagenesis, cDNA synthesis, cDNA cloning and primer extension
- Efficient Reverse Transcription of Templates with High Secondary Structure
- DNA polymerization using template DNA, RNA or RNA:DNA hybrids
- Requires a primer as well as magnesium or manganese
- Stable at higher reaction temperatures (37-58°C)
- Supplied at a concentration of 10u/microl (Cat.# M5101, M5108) or 20-25u/µl (Cat.# M9004)
Avian Myeloblastosis Virus Reverse Transcriptase (AMV RT) catalyzes DNA polymerization using template DNA, RNA or RNA:DNA hybrids. It requires a primer (DNA primers are more efficient than RNA primers) as well as Mg(2+) or Mn(2+). The enzyme possesses an intrinsic RNase H activity. Both nonionic detergents and sulfhydryl compounds stabilize the enzyme activity in vitro.