sparQ Universal Library Quant Kit provides rapid and accurate quantification of libraries prepared for sequencing on Illumina® NGS platforms.

• Faster time to results – 50% shorter run time than traditional cycling protocols
• Accurate, reliable quantification of NGS libraries of various sizes and GC-content
• High amplification efficiency across a wide linear dynamic range
• Stabilized, ready-to-use sparQ Universal Fast Mastermix to reduce pipetting steps
• Superior run to run uniformity ensuring highly precise measurements

Accurate quantification of the number of amplifiable library molecules prior to loading onto a flow cell is a critical step in the NGS workflow and it ensures optimal cluster generation and cost-effective use of sequencing capacity. The sparQ Universal Library Quant Kit uses real-time quantitative PCR (qPCR) to specifically quantify the number of library molecules that possess the appropriate adapter tag at each end.

Accurate library quantification in 40 minutes, optimized for use on any qPCR instrument andreduces qPCR run time by 50%. Complete library quantification solution with unmatched convenience, supplied with all necessary, ready-to-use reagents to minimize pipetting steps, fast cycling protocols work for both 10 μl or 20 μl reaction volumes and reaction setup and data analysis templates and guides provided.

qPCR only quantifies library fragments with adapters attached at both ends. Other methods can lead to overestimation of library concentration. Equivalent performance across cyclers: sparQ vs Kapa Library Quantification Kit. Concordant library Quantification and works with all cylcer and types of nucleic acids. Equivalent performance with 50% faster run time. Highly correlated library quantification results obtained. 50% faster run time compared to Roche KAPA Library Quant Kit. Lot-to-lot consistency of sparQ DNA Standards. High consistency of sparQ DNA Standards. Rigorous quality control to ensure consistent, reliable performance.