LC & LC/MS single-component organic standard.

SPEX CertiPrep creates single and multi-component standards suitable for all HPLC detectors. Our standards are created in HPLC and LC/MS grade solvents to reduce potential interference and increase analytical accuracy.

High Performance Liquid Chromatography (HPLC) is a column-based chromatographic technique used to separate components in a mixture for identification and quantification. A pump system passes pressurized mobile phase and sample through a pre-filled column containing a packing or sorbent which aids in the separation of component peaks. As each analyte in the mixture interacts with the column material, it retards the flow of the analytes within the column. HPLC can be used for identification and quantification of compounds in a variety of fields from agriculture to medical and manufacturing.

A minimal HPLC instrument configuration includes a sampler, pumps, column compartment, and a detector. There are a multitude of various detectors commonly in use with a HPLC system including: Refractive Index Detector (RID), UV/Vis Detector, Photoiode Array Detector, and Mass Spectrometers. Each of these detectors have their own range of use and sensitivity, from the broad range RID detector with its higher detection limits, to the powerful and sensitive LC/MS techniques used for extremely low levels of detection.