Human Recombinant TNF-alpha (from HEK293)
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Supplier: ACRO Biosystems
About this item
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- Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
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Product Details & Documents
Specifications for Product Family
Specifications
Specifications
- Catalog No:
- 103737-588
- 103015-544
- Protein/Peptide Name:
- TNF-alpha
- TNF-alpha
- Protein Synonyms:
- DIF|TNFα|cachexin|cachectin|TNF-alpha|TNFSF2|TNFA
- DIF|TNFα|cachexin|cachectin|TNF-alpha|TNFSF2|TNFA
- Protein/Peptide Type:
- Recombinant
- Recombinant
- Species:
- Human
- Human
- Source:
- HEK293 cells
- HEK293 cells
- Conjugation:
- Unconjugated
- Unconjugated
- Gene ID:
- P01375-1
- P01375-1
- Protease-free:
- Y
- Y
- Animal-Free:
- Y
- Y
- Nuclease-free:
- Y
- Y
- Carrier-Free:
- Y
- Y
- Endotoxin Level:
- Low
- Low
- Endotoxin Content:
- Less than 0.1 EU per μg by the LAL method.
- Less than 0.1 EU per μg by the LAL method.
- Biological Activity:
- Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.; Measured by its binding ability in a functional ELISA. Immobilized ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (HPLC-verified) (Cat. No. TNA-H4211) at 2 μg/mL (100 μL/well) can bind Humira with a linear range of 0.2-2 ng/mL (QC tested).; Measured by its binding ability in a functional ELISA. Immobilized ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (HPLC-verified) (Cat. No. TNA-H4211) at 2 μg/mL (100 μL/well) can bind Human TNFR1, Fc Tag (Cat. No. TN1-H5251) with a linear range of 0.2-3 ng/mL (Routinely tested).; Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (Cat. No. TNA-H4211) with an affinity constant of 0.255 nM as determined in a SPR assay (Biacore T200) (Routinely tested).; ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (Cat. No. TNA-H4211) induces cytotoxicity effect on the WEH1-13VAR cells in the presence of the metabolic inhibitor actinomycin D. The EC50 for this effect is 0.007-0.014 ng/mL (Routinely tested).; Neutralization assay shows that the cytotoxicity effect of ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (Cat. No. TNA-H4211) was inhibited by increasing concentration of Adalimumab. The concentration of TNF-alpha used is 1 ng/mL. The IC50 is 29 ng/mL (Routinely tested).
- Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.; Measured by its binding ability in a functional ELISA. Immobilized ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (HPLC-verified) (Cat. No. TNA-H4211) at 2 μg/mL (100 μL/well) can bind Humira with a linear range of 0.2-2 ng/mL (QC tested).; Measured by its binding ability in a functional ELISA. Immobilized ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (HPLC-verified) (Cat. No. TNA-H4211) at 2 μg/mL (100 μL/well) can bind Human TNFR1, Fc Tag (Cat. No. TN1-H5251) with a linear range of 0.2-3 ng/mL (Routinely tested).; Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (Cat. No. TNA-H4211) with an affinity constant of 0.255 nM as determined in a SPR assay (Biacore T200) (Routinely tested).; ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (Cat. No. TNA-H4211) induces cytotoxicity effect on the WEH1-13VAR cells in the presence of the metabolic inhibitor actinomycin D. The EC50 for this effect is 0.007-0.014 ng/mL (Routinely tested).; Neutralization assay shows that the cytotoxicity effect of ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (Cat. No. TNA-H4211) was inhibited by increasing concentration of Adalimumab. The concentration of TNF-alpha used is 1 ng/mL. The IC50 is 29 ng/mL (Routinely tested).
- Purity:
- >95%
- >95%
- Formulation:
- Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
- Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
- Molecular Weight:
- 17.4 kDa
- 17.4 kDa
- Storage Conditions:
- –20 °C
- –20 °C
- Shipping Temperature:
- Powder,RT
- Powder,RT
Specifications
Product Family Options
Product Information
- SizeAvailabilityPrice
- 25 µgDiscontinued-Supplier Number: TNAH421125UGItem requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:Marketsource
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Specifications
Cat. No.103737-588Protein/Peptide NameTNF-alphaProtein SynonymsDIF|TNFα|cachexin|cachectin|TNF-alpha|TNFSF2|TNFAProtein/Peptide TypeRecombinantSpeciesHumanSourceHEK293 cellsConjugationUnconjugatedGene IDP01375-1Protease-freeYAnimal-FreeYNuclease-freeYCarrier-FreeYEndotoxin LevelLowEndotoxin ContentLess than 0.1 EU per μg by the LAL method.Biological ActivityLyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.; Measured by its binding ability in a functional ELISA. Immobilized ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (HPLC-verified) (Cat. No. TNA-H4211) at 2 μg/mL (100 μL/well) can bind Humira with a linear range of 0.2-2 ng/mL (QC tested).; Measured by its binding ability in a functional ELISA. Immobilized ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (HPLC-verified) (Cat. No. TNA-H4211) at 2 μg/mL (100 μL/well) can bind Human TNFR1, Fc Tag (Cat. No. TN1-H5251) with a linear range of 0.2-3 ng/mL (Routinely tested).; Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (Cat. No. TNA-H4211) with an affinity constant of 0.255 nM as determined in a SPR assay (Biacore T200) (Routinely tested).; ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (Cat. No. TNA-H4211) induces cytotoxicity effect on the WEH1-13VAR cells in the presence of the metabolic inhibitor actinomycin D. The EC50 for this effect is 0.007-0.014 ng/mL (Routinely tested).; Neutralization assay shows that the cytotoxicity effect of ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (Cat. No. TNA-H4211) was inhibited by increasing concentration of Adalimumab. The concentration of TNF-alpha used is 1 ng/mL. The IC50 is 29 ng/mL (Routinely tested).Purity>95%FormulationLyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.Molecular Weight17.4 kDaStorage Conditions–20 °CShipping TemperaturePowder,RTMarketsource - Supplier Number: TNAH42111MGSpecifications:Marketsource
More
Specifications
Cat. No.103015-544Protein/Peptide NameTNF-alphaProtein SynonymsDIF|TNFα|cachexin|cachectin|TNF-alpha|TNFSF2|TNFAProtein/Peptide TypeRecombinantSpeciesHumanSourceHEK293 cellsConjugationUnconjugatedGene IDP01375-1Protease-freeYAnimal-FreeYNuclease-freeYCarrier-FreeYEndotoxin LevelLowEndotoxin ContentLess than 0.1 EU per μg by the LAL method.Biological ActivityLyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.; Measured by its binding ability in a functional ELISA. Immobilized ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (HPLC-verified) (Cat. No. TNA-H4211) at 2 μg/mL (100 μL/well) can bind Humira with a linear range of 0.2-2 ng/mL (QC tested).; Measured by its binding ability in a functional ELISA. Immobilized ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (HPLC-verified) (Cat. No. TNA-H4211) at 2 μg/mL (100 μL/well) can bind Human TNFR1, Fc Tag (Cat. No. TN1-H5251) with a linear range of 0.2-3 ng/mL (Routinely tested).; Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (Cat. No. TNA-H4211) with an affinity constant of 0.255 nM as determined in a SPR assay (Biacore T200) (Routinely tested).; ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (Cat. No. TNA-H4211) induces cytotoxicity effect on the WEH1-13VAR cells in the presence of the metabolic inhibitor actinomycin D. The EC50 for this effect is 0.007-0.014 ng/mL (Routinely tested).; Neutralization assay shows that the cytotoxicity effect of ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (Cat. No. TNA-H4211) was inhibited by increasing concentration of Adalimumab. The concentration of TNF-alpha used is 1 ng/mL. The IC50 is 29 ng/mL (Routinely tested).Purity>95%FormulationLyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.Molecular Weight17.4 kDaStorage Conditions–20 °CShipping TemperaturePowder,RTMarketsource
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