Specifications

• Protein/Peptide Type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Storage Conditions:
  –20 °C
• Endotoxin Content:
  Less than 1.0 EU per μg by the LAL method.
• Biological Activity:
  Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.; Measured by its binding ability in a functional ELISA. Immobilized Human PD-L1, Fc Tag (Cat. No. PD1-H5258) at 5 μg/mL (100 μL/well) can bind Biotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1) with a linear range of 0.02-0.32 μg/mL (QC tested).; Measured by its binding ability in a functional ELISA. Immobilized Nivolumab at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1) with a linear range of 0.2-6 ng/mL (Routinely tested).; Measured by its binding ability in a functional ELISA. Immobilized Biotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Mouse PD-L2, His Tag (Cat. No. PD2-M52E3) with a linear range of 20-156 ng/mL (Routinely tested).; Serial dilutions of nivolumab were added into Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (HPLC-verified) (Cat. No. PD1-H52A3): Biotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1) binding reactions. The half maximal inhibitory concentration (IC50) is 0.3789 μg/mL (Routinely tested).; Serial dilutions of nivolumab were added into Human PD-L2, Mouse IgG1 Fc Tag (Cat. No. PD2-H52A5): Biotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1) binding reactions. The half maximal inhibitory concentration (IC50) is 0.2823 μg/mL (Routinely tested).; Loaded Biotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1) on SA Biosensor, can bind Human PD-L1, His Tag (HPLC verified) (Cat. No. PD1-H5229) with an affinity constant of 2.5 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).; Loaded Biotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1) on SA Biosensor, can bind Human PD-L2, His Tag (SPR verified) (Cat. No. PD2-H5220) with an affinity constant of 0.31 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• Gene ID:
  Q15116-1
• Carrier-Free:
  Y
• Protease-free:
  Y
• Animal-Free:
  Y
• Protein Synonyms:
  CD279|PDCD1|PD1|SLEB2
• Protein/Peptide Name:
  PD-1
• Purity:
  >95%
• Molecular Weight:
  44.4 kDa
• Endotoxin Level:
  Low
• Formulation:
  Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Nuclease-free:
  Y
• Shipping Temperature:
  Powder,RT