Specifications

• Conjugation:
  Unconjugated
• Protein/Peptide Type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Storage Conditions:
  –20 °C
• Endotoxin Content:
  Less than 0.1 EU per μg by the LAL method.
• Biological Activity:
  Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.; Measured by its binding ability in a functional ELISA. Immobilized Human B7-1, Fc Tag (Cat. No. B71-H5259) at 2 μg/mL (100 μL/well) can bind Human CTLA-4, mouse IgG2a Fc tag, low endotoxin (Cat. No. CT4-H52A4) with a linear range of 0.1-2 ng/mL (QC tested).; Measured by its binding ability in a functional ELISA. Immobilized Human B7-2, Fc Tag (Cat. No. CD6-H5257) at 2 μg/mL (100 μL/well) can bind Human CTLA-4, mouse IgG2a Fc tag, low endotoxin (Cat. No. CT4-H52A4) with a linear range of 0.5-7.8 ng/mL (Routinely tested).; Measured by its binding ability in a functional ELISA. Immobilized Ipilimumab at 5 μg/mL (100 μL/well) can bind Human CTLA-4, mouse IgG2a Fc tag, low endotoxin (Cat. No. CT4-H52A4) with a linear range of 0.1-0.8 ng/mL (Routinely tested).; Loaded Human CTLA-4, mouse IgG2a Fc tag, low endotoxin (Cat. No. CT4-H52A4) on Protein A Biosensor, can bind Human B7-1, His Tag (Cat. No. B71-H5228) with an affinity constant of 0.52 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).; Loaded Human CTLA-4, mouse IgG2a Fc tag, low endotoxin (Cat. No. CT4-H52A4) on Protein A Biosensor, can bind Human B7-2, His Tag (Cat. No. CD6-H5223) with an affinity constant of 1.6 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• Gene ID:
  P16410-1
• Carrier-Free:
  Y
• Protease-free:
  Y
• Animal-Free:
  Y
• Protein Synonyms:
  CTLA4|CD152
• Protein/Peptide Name:
  CTLA-4
• Purity:
  >95%
• Molecular Weight:
  40.6 kDa
• Endotoxin Level:
  Low
• Formulation:
  Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Nuclease-free:
  Y
• Shipping Temperature:
  Powder,RT