Human Recombinant CD40 (from HEK293)
- Conjugation:Unconjugated
- Protein/Peptide Type:Recombinant
- Source:HEK293 cells
- Species:Human
- Storage Conditions:–20 °C
- Endotoxin Content:Less than 0.1 EU per μg by the LAL method.
- Biological Activity:Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.; Measured by its binding ability in a functional ELISA. Immobilized Human CD40 Ligand, Fc Tag (Cat. No. CDL-H5267) at 5 μg/mL (100 μL/well) can bind Human CD40, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD0-H525a) with a linear range of 20-156 ng/mL (QC tested).; Loaded Human CD40, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD0-H525a) on Protein A Biosensor, can bind Human CD40 Ligand, His,Flag Tag (active trimer) (MALS verified) (Cat. No. CDL-H52Db) with an affinity constant of 0.911 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).; FACS analysis shows that Human CD40, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD0-H525a) can bind to 293T cells overexpressing human CD40L. The concentration of Human CD40 is 0.03 μg/ml (Routinely tested).
- Gene ID:P25942-1
- Carrier-Free:Y
- Protease-free:Y
- Animal-Free:Y
- Protein Synonyms:p50|CD40|MGC9013|Bp50|TNFRSF5|CDW40
- Protein/Peptide Name:CD40
- Purity:>95%
- Molecular Weight:46.1 kDa
- Endotoxin Level:Low
- Formulation:Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
- Nuclease-free:Y
- Shipping Temperature:Powder,RT
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