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Human Recombinant CD40 (from HEK293)
 
Human Recombinant CD40 (from HEK293)

 

  • Conjugation:
    Unconjugated
  • Protein/Peptide Type:
    Recombinant
  • Source:
    HEK293 cells
  • Species:
    Human
  • Storage Conditions:
    –20 °C
  • Endotoxin Content:
    Less than 0.1 EU per μg by the LAL method.
  • Biological Activity:
    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.; Measured by its binding ability in a functional ELISA. Immobilized Human CD40 Ligand, Fc Tag (Cat. No. CDL-H5267) at 5 μg/mL (100 μL/well) can bind Human CD40, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD0-H525a) with a linear range of 20-156 ng/mL (QC tested).; Loaded Human CD40, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD0-H525a) on Protein A Biosensor, can bind Human CD40 Ligand, His,Flag Tag (active trimer) (MALS verified) (Cat. No. CDL-H52Db) with an affinity constant of 0.911 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).; FACS analysis shows that Human CD40, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD0-H525a) can bind to 293T cells overexpressing human CD40L. The concentration of Human CD40 is 0.03 μg/ml (Routinely tested).
  • Gene ID:
    P25942-1
  • Carrier-Free:
    Y
  • Protease-free:
    Y
  • Animal-Free:
    Y
  • Protein Synonyms:
    p50|CD40|MGC9013|Bp50|TNFRSF5|CDW40
  • Protein/Peptide Name:
    CD40
  • Purity:
    >95%
  • Molecular Weight:
    46.1 kDa
  • Endotoxin Level:
    Low
  • Formulation:
    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
  • Nuclease-free:
    Y
  • Shipping Temperature:
    Powder,RT

 

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