Specifications

• Conjugation:
  Unconjugated
• Protein/Peptide Type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Storage Conditions:
  –20 °C
• Endotoxin Content:
  Less than 0.1 EU per μg by the LAL method.
• Biological Activity:
  Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.; Measured by its binding ability in a functional ELISA. Immobilized FMC63 at 1 μg/mL (100 μL/well) can bind Human CD19 (20-291), Fc Tag, low endotoxin (Super affinity) (Cat. No. CD9-H5251) with a linear range of 1-31 ng/mL (QC tested).; Measured by its binding ability in a functional ELISA. Immobilized Human CD19 (20-291), Fc Tag, low endotoxin (Super affinity) (Cat. No. CD9-H5251) at 5 μg/mL (100 μL/well) can bind FMC63 (Mouse IgG2a) with a linear range of 0.6-5 ng/mL (Routinely tested).; Human CD19 (20-291), Fc Tag, low endotoxin (Super affinity) (Cat. No. CD9-H5251) captured on CM5 chip via Anti-Human lgG Fc antibodies surface, can bind FMC63 MAb (mouse lgG2a) with an affinity constant of 0.17 nM as determined in a SPR assay (Biacore T200) (Routinely tested).; Loaded Human CD19 (20-291), Fc Tag, low endotoxin (Super affinity) (Cat. No. CD9-H5251) on AHC Biosensor, can bind FMC63 MAb (mouse lgG2a) with an affinity constant of 0.483 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).; 293 cells were transfected with FMC63-scFv and RFP tag. 2e5 of the cells were first stained with B. Human CD19 (20-291) Protein, Fc Tag, low endotoxin (Super affinity) (Cat. No. CD9-H5251, 3 µg/ml) and C. Human Fc Tag Protein Control, followed by FITC-conjugated Anti-human IgG Fc Antibody. A. Non-transfected 293 cells and C. Human Fc Tag Protein Control were used as negative control. RFP was used to evaluate CAR (anti-CD19-scFv) expression and FITC was used to evaluate the binding activity of Human CD19 (20-291) Protein, Fc Tag, low endotoxin (Super affinity) (Cat. No. CD9-H5251).
• Gene ID:
  P15391-1
• Carrier-Free:
  Y
• Protease-free:
  Y
• Animal-Free:
  Y
• Protein Synonyms:
  CVID3|MGC12802|B4|CD19
• Protein/Peptide Name:
  CD19
• Purity:
  >90%
• Molecular Weight:
  56.6 kDa
• Endotoxin Level:
  Low
• Formulation:
  Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Nuclease-free:
  Y
• Shipping Temperature:
  Powder,RT