Optimize the plating, passaging and freezing of cell cultures with Cell Systems Reagents. Growth supplements and antibiotic products are also available to ensure each experiment is initiated in an optimal environment.

• High quality specialized reagents
• Always available
• Overnight delivery

The Passage Reagent Group™ (PRG) is a matched set of Cell Systems Certified™ reagents for releasing cells from culture for subculture or freezing. The PRG contains three parts: PRG-1™ (EDTA-dPBS Solution), PRG-2™ (Trypsin/EDTA-dPBS Solution) and PRG-3™ (Trypsin Inhibitor-dPBS Solution). The chelating agent EDTA in PRG-1™ prepares for PRG-2™, which contains highly purified trypsin. PRG-3™ inactivates the protease in PRG-2™ and stabilizes the cell membranes.

Cell membranes are materially and cumulatively damaged whenever cells are exposed to serine proteases, physically manipulated, centrifuged, and/or frozen. Use of the PRG greatly minimizes damage and stress to cells during passage or freezing of cell cultures.

PRG-3™ is intended for sequential use with PRG-1™ and PRG-2™. PRG-3™ inhibits the trypsin activity of PRG-2™, preventing nonspecific protease damage to cell membranes after detachment.

Instructions for use:
Click to view the Passage Reagent Group protocol.
Handling and Storage:
Store at －20 °C. Once opened, shelf life is 30 days at 2 to 8 °C.