Optimize the plating, passaging and freezing of cell cultures with Cell Systems Reagents. Growth supplements and antibiotic products are also available to ensure each experiment is initiated in an optimal environment.

• High quality specialized reagents
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The Passage Reagent Group™ (PRG) is a matched set of Cell Systems Certified™ reagents for releasing cells from culture for subculture or freezing. The PRG contains three parts: PRG-1™ (EDTA-dPBS Solution), PRG-2™ (Trypsin/EDTA-dPBS Solution) and PRG-3™ (Trypsin Inhibitor-dPBS Solution). The chelating agent EDTA in PRG-1™ prepares for PRG-2™, which contains highly purified trypsin. PRG-3™ inactivates the protease in PRG-2™ and stabilizes the cell membranes.

Cell membranes are materially and cumulatively damaged whenever cells are exposed to serine proteases, physically manipulated, centrifuged, and/or frozen. Use of the Passage Reagent Group (PRG) greatly minimizes damage and stress to cells during passage or freezing of cell cultures.

PRG-2 (Trypsin-EDTA Solution 1×), 100 ml contains highly purified trypsin, allowing a substantial reduction (40%) in the amount of enzyme required to detach cells compared to typical commercial trypsin solutions. Intended for sequential use with PRG1™ (EDTA-dPBS Solution) and PRG3™ (Trypsin Inhibitor-dPBS solution).

Instructions for use:
Click to view the Passage Reagent Group protocol.
Handling and Storage:
Store at －20 °C. Once opened, shelf life is 30 days at 2 to 8 °C.