To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.
Specifications
- Description:Cell culture media
- Size:500 ml
- Environmentally Preferable:
- Cat. No.:103810-616
Specifications
About this item
Our suite of specialized media provides our customers with a diverse set of tools to enhance research through consistent and stable environments for cell cultures. Cell Systems media may be used with all primary, immortalized, and established cell lines.
- Based on a modified and optimized basal DMEM/F12 (1:1)
- Contain serum at 10% by volume
- Do not contain phenol red
- Packaged in 500 ml bottles (as a kit)
Medium is formulated with 10% serum. Kit consists of 500mL Cell Systems Medium (4Z0-500-R), 10 ml CultureBoost-R™(containing human recombinant growth factors) and 10 ml Attachment Factor™ (extracellular matrix reagent for coating flasks). This becomes a complete medium once activated with the included CultureBoost-R™ supplement. 4Z0-500-R is Certified and intended for experimental application.
Medium contains no added hormones. Medium contains no antibiotics; however, Bac-Off® (Cat. #4Z0-643), is the recommended antibiotic for all Cell Systems medium. Bac-Off® is used at or above the 2 µg/ml MIC of aerobic Gram-positive and Gram-negative microorganisms.
Medium contains no phenol red. Phenol red in cell culture medium binds to receptors on cell surfaces and causes the cell to respond as though pharmacologic levels of estrogen and/or inflammatory mediators (such as prostaglandin or thromboxane A2) are present in the medium. We exclude this unnecessary additive in order to optimize experimental conditions for researchers.
Certified for use with more than 100 human and animal cell cultures and cell lines including:
Human: All Cell Systems Primary Cells; Bone Marrow Cells (CFU-F and Erythroid Burst Cultures); Connective Tissue Cells; Dermal Fibroblast Cells; Endothelial Cells (Aortic, Arterial, Coronary Artery, Vascular and Venous); Fetal Lung Cells; Embryonic and Adult Stem Cells; Fibroblast Cells; Glomerular Mesangial Cells; Immortalized / Tumor-derived Mesenchymal Cells; Microvascular Cells (Cerebral, Coronary, Dermal, Glomerular, Liver, Lung and Retinal); Smooth Muscle Cells; Tumor Neovascular Cells.
Medium Storage:Cell Systems Medium is shipped at ambient temperature. If the entire unit will not be used within 30 days, supplement the medium with CultureBoost-R™, then aliquot and freeze in smaller units which will be used within 30 days. Otherwise, immediately refrigerate the medium and supplement. Once the unit is supplemented, or any component of the medium kit is opened, the shelf life is 30 days at refrigerated (2 to 8 °C) temperatures.
For best results, use Cell Systems Medium in conjunction with cell culture vessels coated with Attachment Factor™ extracellular matrix reagent.