Tyramide Amplification Kit with HRP Goat, Anti-Rabbit and CF® Dye or Biotin Tyramide, Biotium

Kits for increasing immunofluorescence sensitivity using HRP-catalyzed tyramide signal amplification with the choice of next-generation fluorescent CF® dyes or biotin.

Increase sensitivity by up to 100-fold
Use lower primary antibody concentrations
Boost signal in challenging samples, like human FFPE tissues
Choose from six bright and photostable CF® dyes or biotin

Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to conventional avidin-biotin detection. Multiple rounds of tyramide signal amplification can be performed for multiplex detection.

The HRP goat anti-rabbit antibody provided in the kits has been adsorbed against human, mouse, and rat serum to minimize cross-reactivity. The kits include choice of six CF® dye tyramides or biotin tyramide. CF® dyes are line of next-generation fluorescent dyes, with advantages in brightness and photostability compared to other dyes.

: Each kit contains reagents for staining 50 to 150 slides; the actual number of samples that can be stained will depend on staining volumes used. Tyramide amplification buffer, CF® dye tyramides, and other tyramide compounds are also available separately. Note: Endogenous biotin blocking reagents (not provided) may be required for streptavidin/biotin detection in cells or tissues.

: Kit components: CF® dye or biotin-tyramide stock solution, HRP goat anti-rabbit IgG (highly cross-adsorbed), BSA for blocking, Tyramide amplification buffer plus, 30% Hydrogen peroxide.