- Protein/Peptide Type:Recombinant
- Source:CHO cells
- Species:Human
- Size:10 µg
- Storage Conditions:2...8 °C (1 month from the date of receipt);–20...–70 °C (3 - 12 months from the date of receipt)
- Endotoxin Content:<0.01 EU per 1 μg of the protein by the LAL method
- Biological Activity:Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.4 - 1.6 ng/ml
- Form:Disulfide-linked homodimer
- Reconstitution Instructions:It is recommended that sterile 4 mM HCl be added to the vial to prepare a working stock solution of no less than 100 μg/ml. The carrier-free protein should be used immediately upon dilution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.
- Carrier-Free:Y
- Protein Synonyms:BMP9|BMP9BMP-9Bone morphogenetic protein 9|GDF-2|GDF2|growth differentiation factor 2|growth/differentiation factor 2
- Accession No.:Q9UK05
- UniProtKB:Q9UK05
- Protein/Peptide Name:BMP-9
- Purity:>95%
- Molecular Weight:12.1 kDa (Monomer)
- Sequence:Ser320
- Formulation:Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA
- Tested Applications:Bioactivity
- Purification:SDS-PAGE under reducing conditions and visualized by silver stain
- Cat. No.:103710-716
Human BMP-9, also known as growth and differentiation factor 2 (GDF-2), is a member of the BMP subgroup of the TGF-beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors.
- Carrier-free
BMP-9 regulates the development and function of a variety of embryonal and adult tissues. The human BMP-9 cDNA encodes a 429 amino acid (aa) precursor that includes a 22 aa signal sequence, a 298 aa propeptide, and a 111 aa mature protein. The propeptide does not interfere with the biological activity of BMP-9 and remains associated with the mature peptide after proteolytic cleavage.
Human and mouse BMP-9 share 96% aa sequence identity. Within the mature protein, human BMP-9 shares 64% aa sequence identity with human BMP-10 and less than 50% aa sequence identity with other BMPs. BMP-9 is expressed by non-parenchymal cells in the liver, where it promotes lipid metabolism and inhibits glucose production.
BMP-9 exerts a prolonged hypoglycemic effect which may be due to an enhancement of insulin release. BMP-9 interacts with a high affinity specific heteromeric receptor expressed on liver endothelial cells that has been identified as ALK-1 (4 - 6). In the embryonal CNS, BMP-9 functions in the development and maintenance of the cholinergic neuronal phenotype. BMP-9 also induces the differentiation of mesenchymal stem cells into the chondrogenic lineage. At low concentrations, BMP-9 is a proliferative factor for hematopoietic progenitor cells, but at higher concentrations, it enhances TGF-beta 1 production and inhibits hematopoietic progenitor colony formation.