- Antibody Type:Primary
- Antigen Name:Brain-derived neurotrophic factor, Beta-nerve growth factor, Neurotrophin-3, Neurotrophin-4
- Antigen Symbol:BDNF, NGF, NTF3, NTF4
- Clonality:Polyclonal
- Conjugation:Unconjugated
- Host:Sheep
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG
- Reactivity:Human,Rat,Mouse
- Western Blot:Yes
- Size:5 × 500 µg
- Neutralisation:Yes
- Form:Lyophilized
- Gene ID:BDNF, Ngf, NTF3, NTF4
- Storage Temperature:Store lyophilised, unopened vials at 2…8° C or lower. After reconstitution, prepare aliquots and store at -20° C for a higher stability. Avoid freeze-thaw cycles.
- Shipping Temperature:Room (ambient) temperature
- Immunogen:BDNF: recombinant human BDNF
NGF: native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
NT3: recombinant human NT3
NT4/5: recombinant human NT4 - Purification:Protein G purified.
- Cat. No.:76333-986
Neurotrophins are growth factors that regulate neuronal development, maintenance and plasticity. These homo-dimeric proteins activate a multitude of intracellular signalling cascades through their interaction with Trk receptors and p75NTR.
The Neurotrophin Antibody Kit contains a collection of Biosensis' popular sheep antibodies raised against BDNF (S-015-500), NGF (S-052-500), NT3 (S-057-500) and NT4/5 (S-059-500). The kit is completed with one vial of normal sheep IgG (S-1754-500) for use as negative control. This full size version of Biosensis' Neurotrophin Antibody Kit contains 500 ug sheep IgG of each antibody.
This kit presents a cost-effective way to investigate neurotrophin protein expression and function by immunological techniques such as immunohistochemistry, ELISA, and inhibition of biological activity.
Applications: Immunohistochemistry: 1-10 ug/mL. BDNF antibody works best on Zamboni's fixed, frozen tissue, and is not recommended for paraffin-embedded tissue. ELISA: 1-10 ug/mL. Western Blotting: 1-10 ug/mL. Western blotting is not recommended for the BDNF antibody.
Inhibition of biological activity in vitro/in vivo: Expected ED50 (in vivo) values are in the range of 2-10 ug/mL. The NGF antibody completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. The negative control (normal sheep IgG) should be used at the same concentration as the neurotrophin antibodies.
Type: Primary
Antigen: BDNF, NGF, NTF3, NTF4
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Sheep
Isotype: IgG
Reactivity: Human, rat, mouse