CELLvo™ Human Mesenchymal Stem Cells- Wharton's Jelly (hMSC-WJ), StemBioSys

Supplier: StemBioSys

Certificates

Supplier: StemBioSys

About this item

CELLvo™ human mesenchymal stem cells-Wharton's Jelly are adult multipotent stem cells cryopreserved at passage two.

Highly proliferative
Smaller cell size
Xeno-free
Higher percentage of SSEA4+ cells
>20 population doublings

Wharton's Jelly is a rich source of highly proliferative, multipotent, mesenchymal stem cells. These cells are known to be highly immunomodulatory and be capable of trilineage differentiation.

CELLvo™ human mesenchymal stem cells- Wharton's Jelly have been isolated and cultured under xeno free conditions on CELLvo™ XF-Matrix. CELLvo™ MSC-WJ proliferate more quickly, have a smaller cell size and show a higher percentage of SSEA4 (+) cells as compared to Wharton's Jellly MSCs isolated and cultured on other substrates.

Thaw cells quickly in a 37 °C waterbath. Approximate thaw time for a 1 ml sample is 2 to 3 minutes. Do not allow the cell vial to warm above chilled temperatures. Dilute cell/CryoStor® mixture in an appropriate volume of cell culture media and plate cells in the appropriate configuration. Live/dead fluorescsent assays or metabolic assays 24 hours post-thaw are recommended for accurate assessment of cell survival.

Ordering information: CELLvo™ human mesenchymal stem cell - Wharton's Jelly (hMSC-WJ) are derived from a single donor and cryopreserved at P2. CELLvo™ hMSC-WJ can be ordered as a single vial of cells or as a kit to include CELLvo™ XF matrix.

Delivery: A vial of cells will include >1 million CELLvo™ hMSC-WJ. A kit will contain a single vial of hMSC-WJ croypreserved at passage 2 and a sleeve of CELLvo™ XF Matrix (6 well plates, T 75 flasks or T 150 flasks). Additional CELLvo™ XF matrix can be purchased separately.

Packaging: CELLvo™ hMSC-WJ arrive on dry ice and may be purchased to include a sleeve of CELLvo™ XF matrix. hMSC-WJ should be stored in liquid nitrogen vapor upon arrival. CELLvo™ XF matrix should be stored at 2 to 8 °C upon arrival. Avoid extended exposure to light.

Caution: CELLvo™ cells are cryopreserved in CryoStor®solution. To obtain an accurate measure of cell viability following cryopreservation, assesment should be performed 24 hours post thaw and compared to non-frozen controls. Sample assesment immediately post-thaw with membrane integrity indicators such as Trypan Blue will often result in inaccurate measurement of cell survival. Live/dead fluorescsent assays or metabolic assays are recommended for a more accurate assessment of cell survival.

CELLvo™ products are not for human use. These products should not be used for diagnostic or therapeutic purposes. Handle in accordance with established bio-safety practices.

Restricted Products: To process your orders without delay, please provide the required business documentation to purchase this product.

To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:

• State issued document with your organization's Federal Tax ID Number
• State issued document with your organization's Resale Tax ID Number
• City or County issued Business License
• State Department of Health Services License
• Any other ID issued by the State that includes the business name & address

* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.

Specifications

Storage Conditions:
Upon arrival, store cells in liquid nitrogen vapor
Storage Buffer:
CryoStor®CS5
Tested Applications:
Multilineage differentiation, flow cytometry and viability