The FastMitophagy™ detection kit is specifically designed for mitophagy detection in mammalian cells.

• Store at 0 to 5 °C with protection from light

The kit is composed of a mitophagy dye, a reagent for detection of mitophagy, and lysosome dye. Reagents are sufficient for 5×96 well plates or 25 assays when using a 35 mm dish. Mitophagy dye initially accumulates in intact mitochondria and becomes immobilized via chemical bonding, resulting in a very weak fluorescence due to the influence of the surrounding environment.

When mitophagy is induced, the damaged mitochondria fuses to lysosome and the Mitophagy dye emits a high fluorescence. Lyso dye is used to confirm the fusion of Mitophagy dye - labeled mitochondria and lysosome.