igScript™ RT-qPCR kit requires minimal handling during reaction setup and offers consistent and robust RT-qPCR reactions.

• Robust and active for cDNA synthesis at temperatures up to 55 °C
• Highly efficient at producing full-length cDNA from as little as 50 pg of total RNA
• Useful for cDNA synthesis followed by gene expression data validation
• Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product
• 5X igScript™ master mix
• Oligo d(T)23 VN primer (50 µM)
• Random hexamer primer mix (60 µM)
• ig SYBR® Green qPCR 2X master mix
• Nuclease free water

igScript™ one step RT-qPCR kit combines two powerful mixtures: igScript™ reverse transcriptase and ig SYBR® green qPCR 2X master mix with standard buffer providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity. The two mixtures require minimal handling during reaction setup and offer consistent and robust RT-qPCR reactions.

igScript™ reverse transcriptase is a recombinant MMLV reverse transcriptase with reduced RNase H activity and increased thermostability. The kit is highly efficient at producing full-length cDNA from long RNA templates at temperatures between 42 to 55 °C.

ig™ SYBR green qPCR 2X master mix is a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA in qPCR. The ig SYBR® green qPCR 2X master mix with integrated chemically-modified hot start Taq polymerase, SYBR® green I fluorescent dye, ROX dye,MgCl₂, dNTPs and stabilizers. The amplification step features a high quality hot start Taq DNA polymerase which offers higher fidelity and better amplification.