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- Size:5000 units
- Enzyme Name:T5 Exonuclease
- Concentration:10 U/µl
- Cat. No.:103753-178
T5 Exonuclease is a highly efficient 5' - 3' exonuclease for either ssDNA or dsDNA.
- Useful for plasmid mutagenesis methods, oligonucleotide site-directed mutagenesis, generation of plasmid-sequencing templates, generation of 3'-overhang for improved cloning procedures
- Extremely high purity
- Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product
- The physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie® blue staining
- 10X T5 exonuclease reaction buffer
T5 exonuclease is a highly efficient 5' - 3' exonuclease for either ssDNA or dsDNA. It also has ssDNA specific endonuclease activity in the presence of magnesium ions. However, the enzyme does not degrade supercoiled dsDNA . The mode of action of T5 Exonuclease in vivo may be analogous to that of the 5' - 3' exonuclease activity of E. coli DNA Polymerase I.