T5 Exonuclease is a highly efficient 5' - 3' exonuclease for either ssDNA or dsDNA.

• Useful for plasmid mutagenesis methods, oligonucleotide site-directed mutagenesis, generation of plasmid-sequencing templates, generation of 3'-overhang for improved cloning procedures
• Extremely high purity
• Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product
• The physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie® blue staining
• 10X T5 exonuclease reaction buffer

T5 exonuclease is a highly efficient 5' - 3' exonuclease for either ssDNA or dsDNA. It also has ssDNA specific endonuclease activity in the presence of magnesium ions. However, the enzyme does not degrade supercoiled dsDNA . The mode of action of T5 Exonuclease in vivo may be analogous to that of the 5' - 3' exonuclease activity of E. coli DNA Polymerase I.