Taq ligase is thermostable DNA ligase from the thermophilic bacterium Thermus aquaticus cloned to E. coli cells.

• Useful for allele-specific gene detection via LCR and mutagenesis by incorporation of a phosphorylated oligonucleotide
• Extremely high purity
• Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product
• The physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie® blue staining
• 10X Taq DNA ligase buffer with NAD+

Taq DNA ligase catalyzes the formation of a phosphodiester bond in duplex DNA containing adjacent 5'-phosphoryl and 3'-hydroxyl termini, using NAD+ as a cofactor. The ligation will occur only if the oligonucleotides are perfectly paired to the complementary target DNA and have no gaps between them therefore, a single-base substitution can be detected. Taq DNA ligase is active at elevated temperatures (45 to 70°C).