Agrobacterium electro-competent cells provide the highest bacterial transformation efficiency, along with availability of unique strains.
- High bacterial transformation efficiency
- Available as 50 μl vials
- Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product
- Includes AGL1 electro-competent cells, pCAMBIA control DNA and recovery medium
The AGL1 strain (A. tumefaciens) has a C58 chromosomal background that carries an insertion mutation in its recA recombination gene which stabilizes recombinant plasmids. It also carries rifampicin and carbenicilin resistance in its genome for selection. AGL1 contains the Ti plasmid pTiBO542 from which the T-DNA region sequences have been deleted. Transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of Arabidopsis thaliana as well as maize and other monocots.