ZR BAC DNA Miniprep Kit, Zymo Research
Supplier: Zymo Research
Certificates
About this item
The ZR BAC DNA miniprep kit is for efficient isolation of BAC plasmid DNA and other large plasmids (e.g., PAC) from E. coli cell lysates.
- Simple
- Rapid
- User-friendly
- Reliable
- No messy precipitations
Unique column design offers zero buffer retention and low-volume (10 µl) elution. It features a modified alkaline lysis protocol together with unique spin column matrix technology to yield high quality plasmid DNA in minutes and color-coded reagents that allow easy visualization and assessment of complete bacterial cell lysis and buffer neutralization.
The innovative Zymo-Spin™ IC-XL columns are optimized for high yield endotoxin-free plasmid DNA recovery. BAC DNA purified using the ZR BAC DNA miniprep kit is well suited for sequencing, PCR, endonuclease digestion, and more.
Delivery: The kit includes P1 buffer, P2 buffer, P3 buffer, endo-wash buffer, plasmid wash buffer (concentrate), DNA elution buffer, Zymo-Spin™ IC-XL columns, collection tubes and instruction manual.
Specifications
- Method / Format:Column (silica-based)
- Prep size:Mini
- Refine target molecule:Larger vectors (Cosmid, BAC, PAC, Fosmid)
- Sample type:Bacteria
- Target molecule:Plasmid
- Description:ZR BAC DNA miniprep kit
- Yield:Up to 10 µg per preparation, depending on the plasmid copy number, initial volume of E. Coli culture processed, and culture growth conditions. The typical yield is 1 µg per 3 ml of culture.