Human Recombinant TNF alpha (from E. coli)

Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism.

Reconstitute at 0.1 to 1 mg/ml in sterile PBS
Carrier-free

It consists of a 35 amino acid cytoplasmic domain, a 21 amino acid transmembrane segment, and a 177 amino acid extracellular domain (ECD). Within the ECD, it shares 97% sequence identity with rhesus and 71 to 92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha. It is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. It is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface.

Store at –20 to –70 °C as supplied for 12 months and after reconstitution for six months; 2 to 8 °C under sterile conditions after reconstitution for one month.

Protein/Peptide Type:
Recombinant
Source:
E. coli
Species:
Human
Endotoxin Content:
<0.10 EU/μg of protein
Form:
Lyophilized
Carrier-Free:
Y
Animal-Free:
Y
Protein Synonyms:
Cytotoxin|Necrosin|TNFSF1A|TNFSF2|Tumor Necrosis Factor|Differentiation-inducing factor(DIF)|Tumor necrosis factor-alpha|Cachectin
Accession No.:
P01375
Protein/Peptide Name:
TNF alpha
Purity:
>97%
Molecular Weight:
17 kDa
Tested Applications:
Bioassay