About this item
RNA from any sample (cells, tissues, biological fluids and more).
- DNA-free: Genomic DNA removal column and DNase I included
- High quality: Ensure intact RNA with DNA/RNA Shield™ for sample storage at ambient temperatures
- NGS-ready: RNA is ready for all downstream applications including next-gen sequencing, RT-qPCR, hybridization and more
The Quick-RNA™ miniprep plus kit is an innovative and versatile product designed for the easy, reliable, and rapid isolation of DNA-free RNA from cells, all tissue types, whole blood, and biological fluids. The provided DNA/RNA Shield™ stabilizes samples, allowing them to be stored without the need for immediate freezing or processing for up to one month. Furthermore, DNA/RNA Shield™ inactivates RNases as well as microbial pathogens (viruses, bacteria and more). The procedure combines a unique buffer system with Zymo-Spin™ column technology to yield high quality total RNA (including small RNAs 17 to 200 nt).
Simply add DNA/RNA Shield™ and proteinase K to extract total RNA from any tissue, then purify the RNA using the Zymo-Spin™ column. The result is highly-concentrated, DNA-free RNA that is suitable for RT-PCR, hybridization, sequencing and more. In addition, the kit can be used for the enrichment of small and large RNAs in two separate fractions.
Specifications
- Method / Format:Column (silica-based)
- Prep size:Mini
- Refine target molecule:Total RNA
- Sample type:Cultured cellsHuman/animal cells and tissues
- Target molecule:RNA
- Sample Size:≤1×107 Cells or 50 mg Tissue
- Description:Quick-RNA™ Miniprep Plus Kit
- Sample capacity:100 µg
- Yield:spin column, 100 µg RNA (binding capacity), ≥50 µl (elution volume)