About this item
High-Fidelity (HF®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer (CutSmart® Buffer).
- Maintains high activity levels in T4 DNA ligase buffer
- 100% activity in CutSmart Buffer
- Time-Saver™ qualified for digestion in 5 to 15 minutes
Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence.
BsaI-HFv2 has been optimized for Golden Gate Assembly. BsaI-HFv2 also works well for any protocol requiring DNA cutting by BsaI. This is the recommended enzyme for any purpose requiring digestion at the recognition sequence 5'-GGTCTC(N1)/(N5)-3'.
This enzyme is produced from E. coli strain that carries the cloned and modified BsaI gene from Bacillus stearothermophilus 6-55.
To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.
Specifications
- Enzyme Name:BsaI-HF®v2