Specifications
- Protein Function:Cytokines and Growth Factors
- Protein/Peptide Type:Recombinant
- Source:Mouse myeloma cell line
- Species:Human
- Size:10 μg
- Storage Conditions:Store between –70...–20 °C (12 Months)
- Endotoxin Content:<1.0 EU/μg
- Reconstitution Instructions:Reconstitute at 100 μg/ml in Sterile PBS Containing at least 0.1% Human or Bovine Serum Albumin
- Protein Synonyms:IL-27 subunit alpha|IL-27-A|IL27p28|Interleukin-27 subunit alpha|IL27|IL27A|IL-27|p28IL-27A|Interleukin 30|IL-27 p28 subunit|IL30|Interleukin 27|IL27-A|MGC71873
- Protein/Peptide Name:IL-27
- Purity:>90%
- Molecular Weight:50 kDa
- Sequence:Arg21 (N-Terminal)
- Endotoxin Level:Low
- Cat. No.:103703-556
Specifications
About this item
Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus.
- Buffer: Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, EDTA and CHAPS with BSA as a carrier protein
IL-27 is a heterodimeric group 2 receptor ligand molecule that belongs to the IL-6/IL-12 family of long type I cytokines. It is composed of EBI3 (EBV-induced gene 3), a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, the 28 kDa glycoprotein that is related to the p35 chain of IL-12.
The human EBI3 gene encodes a 229 amino acid (aa) precursor that contains a 20 aa signal peptide and 209 aa mature protein. The mature region contains two potential N-linked glycosylation sites, two fibronectin type III domains, and two pairs of conserved cysteine residues with a WSXWS-like motif that places the molecule in the hematopoietin receptor family. Although p40, the EBI3 counterpart in IL-12, is known to form homodimers, there is no evidence to date that EBI3 also homodimerizes. Human EBI3 is 61% aa identical to mouse EBI3. The human p28 gene encodes a 243 aa precursor that contains a 28 aa signal sequence and 215 aa mature region. The mature region is characterized by the presence of four alpha-helices, placing it in the IL-6 family of helical cytokines. Human p28 is 74% aa identical to mouse p28. IL-27 is expressed by monocytes, endothelial cells and dendritic cells.
IL-27 binds to and signals through a heterodimeric receptor complex composed of WSX-1 (TCCR) and gp130. Evidence suggests IL-27 interacts only with WSX-1. IL-27 has both anti- and proinflammatory properties. As an anti-inflammatory, IL-27 seems to induce a general negative feedback program that limits T and NK-T cell activity. At the onset of infection, IL-27 induces an IL-12 receptor on naïve CD4+ T cells, making them susceptible to subsequent IL-12 activity.