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Human Recombinant IL-27 (from Mouse myeloma cell line)
Human Recombinant IL-27 (from Mouse myeloma cell line)
Catalog # 103703-556
Supplier:  Novus Biologicals
CAS Number:  
Human Recombinant IL-27 (from Mouse myeloma cell line)
Catalog # 103703-556
Supplier:  Novus Biologicals
Supplier Number:  2526-IL-010
CAS Number:  

Specifications

  • Protein Function:
    Cytokines and Growth Factors
  • Protein/Peptide Type:
    Recombinant
  • Source:
    Mouse myeloma cell line
  • Species:
    Human
  • Size:
    10 μg
  • Storage Conditions:
    Store between –70...–20 °C (12 Months)
  • Endotoxin Content:
    <1.0 EU/μg
  • Reconstitution Instructions:
    Reconstitute at 100 μg/ml in Sterile PBS Containing at least 0.1% Human or Bovine Serum Albumin
  • Protein Synonyms:
    IL-27 subunit alpha|IL-27-A|IL27p28|Interleukin-27 subunit alpha|IL27|IL27A|IL-27|p28IL-27A|Interleukin 30|IL-27 p28 subunit|IL30|Interleukin 27|IL27-A|MGC71873
  • Protein/Peptide Name:
    IL-27
  • Purity:
    >90%
  • Molecular Weight:
    50 kDa
  • Sequence:
    Arg21 (N-Terminal)
  • Endotoxin Level:
    Low
  • Cat. No.:
    103703-556

Specifications

About this item

Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus.

  • Buffer: Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, EDTA and CHAPS with BSA as a carrier protein

IL-27 is a heterodimeric group 2 receptor ligand molecule that belongs to the IL-6/IL-12 family of long type I cytokines. It is composed of EBI3 (EBV-induced gene 3), a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, the 28 kDa glycoprotein that is related to the p35 chain of IL-12.

The human EBI3 gene encodes a 229 amino acid (aa) precursor that contains a 20 aa signal peptide and 209 aa mature protein. The mature region contains two potential N-linked glycosylation sites, two fibronectin type III domains, and two pairs of conserved cysteine residues with a WSXWS-like motif that places the molecule in the hematopoietin receptor family. Although p40, the EBI3 counterpart in IL-12, is known to form homodimers, there is no evidence to date that EBI3 also homodimerizes. Human EBI3 is 61% aa identical to mouse EBI3. The human p28 gene encodes a 243 aa precursor that contains a 28 aa signal sequence and 215 aa mature region. The mature region is characterized by the presence of four alpha-helices, placing it in the IL-6 family of helical cytokines. Human p28 is 74% aa identical to mouse p28. IL-27 is expressed by monocytes, endothelial cells and dendritic cells.

IL-27 binds to and signals through a heterodimeric receptor complex composed of WSX-1 (TCCR) and gp130. Evidence suggests IL-27 interacts only with WSX-1. IL-27 has both anti- and proinflammatory properties. As an anti-inflammatory, IL-27 seems to induce a general negative feedback program that limits T and NK-T cell activity. At the onset of infection, IL-27 induces an IL-12 receptor on naïve CD4+ T cells, making them susceptible to subsequent IL-12 activity.