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Specifications
- Media Format:Powder
- Description:Tryptic Soy Agar
- Size:500 g
- Cat. No.:IC091010617
- Storage temperature:Room Temperature (15...30 °C)
Specifications
About this item
Tryptic Soy Agar is a medium very rich in nutrients for 'general use' in microbiological laboratories. If desired, antibiotics can easily be incorporated as well as other supplements or inhibitory agents.
- Used for the isolation and cultivation of fastidious, non-fastidious micro-organisms and also for sterility testing
- Lacks carbohydrates
- Useful in the study of hemolytic reactions and also in the preparation of chocolate agar
- Used with or without blood supplement
- Used as precultivation medium and enumeration (E. coli) according to membrane-filter technique
In Tryptic Soy Agar, casein peptone and soya peptone provide nitrogen, vitamins and minerals. The natural sugars from soya peptone promote bacterial growth. Sodium chloride is for the osmotic balance. As it does not contain the X and V factors, it is suitable for identification of Haemophilus sp. by adding X (Hemin) and V (DPN) factors strips
Suspend 40 g of the medium in one liter of deionized or distilled water. Heat with frequent agitation and boil for one minute. Sterilize in an autoclave between 118 and 121 °C at a pressure of not more than 15 lbs. for 15 minutes. In the case of large volume preparation, increase the time of sterilization but not the temperature or pressure. Cool and pour into petri dishes. For hemolytic studies, add 5 to 10% sheep or rabbit blood.
A short list of microorganisms that grow on this medium are the following: Streptococcus, Neisseria, Brucella, Corynebacterium, Listeria, Pasteurella, Vibrio, Haemophilus vaginals, Candida and more.