Potato Dextrose Broth is used for the isolation, enumeration and identification of yeast and molds. It can also be used in the identification of fungi and yeasts in parallel with their cellular morphology or in methods of mirocultivation in slides.

• Suited for the cultivation of a variety of yeasts and molds from dairy products and other foods
• This medium is recommended by the Assocation of Public Health (APHA)
• Analysis of dairy products, bottled drinks, frozen food and other types of food

When the medium is to be used for enumeration of molds and yeasts, add to the medium, sterilized and cooled to 45 to 50 °C, approximately 14 ml of a sterilized 10% solution of tartaric acid to obtain a pH of 3.5.

Suspend 24 g of the medium in one liter of distilled or deionized water. Mix well and heat with frequent agitation. Boil for one minute. Sterilize at 121 °C (15 lbs. of pressure) for 15 minutes.