Nutrient Broth is a general purpose medium used for cultivating a broad variety of fastidious and non-fastidious microorganisms with non-exacting nutritional requirements.

• Growth of fastidious and non-fastidious microorganisms
• Used for enumeration, isolation and enrichment
• High grade base for the preparation of special media
• With the addition of blood, ascites fluid or serum, they can also be used to cultivate streptococci, pneumococci and Erysipelas species and more

Peptone and yeast extract provide nitrogenous compounds, vitamin B complex, amino acids and other essential growth nutrients. Sodium chloride is used to maintain the osmotic balance and glucose is the fermentable carbohydrate source of the media.

This medium is used in accordance with official recommended procedures for the bacteriological analyses of water, milk and dairy products, in foods of important sanitation, tests for sensitivity and resistance and as a base to prepare media supplemented with other nutrients.

Dissolve 8 g of the medium in a liter of deionized or distilled water. Dispense as desired and sterilize at 121 °C (15 lbs. of pressure) for 15 minutes.