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- Size:100 ml
- Enzyme Name:Trypsin
- Cat. No.:IC091689349
Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.
- Trypsin-EDTA solutions are used to release adherent cells from culture surfaces.
- They are generally composed of variable concentrations of natural porcine pancreas-derived trypsin and EDTA.
- Trypsin 1X solutions can range from 0.025% to 0.5%. The reasons for the range of concentrations are differences in required trypsin activity or potency; differences in incubation times; and cell line susceptibility.
- The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.
- Various formulations should be tested to determine the best product for a specific application.