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Human Recombinant SIRP alpha (from HEK293 Cells), Fc Tag
Human Recombinant SIRP alpha (from HEK293 Cells), Fc Tag
Supplier:  ACROBIOSYSTEMS INC MS
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Human Recombinant SIRP alpha (from HEK293 Cells), Fc Tag
Supplier:  ACROBIOSYSTEMS INC MS

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Specifications

  • Conjugation:
    Unconjugated
  • Protein Function:
    Immune Checkpoint Proteins
  • Protein/Peptide Type:
    Recombinant
  • Source:
    HEK293 cells
  • Species:
    Human
  • Tag sequence:
    Fc Tag
  • Storage Conditions:
    –20 °C
  • Endotoxin Content:
    Less than 1.0 EU per μg by the LAL method.
  • Biological Activity:
    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.; Measured by its binding ability in a functional ELISA. Immobilized Human CD47, His Tag (Cat. No. CD7-H5227) at 2 μg/mL can bind Human SIRP alpha, mouse IgG1 Fc Tag (HPLC-verified) (Cat. No. SIA-H52A8) with a linear range of 8-250 ng/mL (QC tested).; FACS assay shows that recombinant Human SIRP alpha, mouse IgG1 Fc Tag (HPLC-verified) (Cat. No. SIA-H52A8) can bind to Jurkat cell expressing CD47. The concentration of SIRP alpha used is 0.3 μg/ml (Routinely tested).; FACS analysis shows that the binding of Human SIRP alpha, mouse IgG1 Fc Tag (HPLC-verified) (Cat. No. SIA-H52A8) to Jurkat expressing CD47 was inhibited by increasing concentration of neutralizing anti-CD47 antibody. The concentration of SIRP alpha used is 0.3 μg/ml. IC50=0.1318 μg/ml (Routinely tested).
  • Protease-free:
    Y
  • Animal-Free:
    Y
  • Protein Synonyms:
    MYD1|PTPNS1|CD172A|SHPS1|P84|SIRPA|BIT|MFR
  • UniProtKB:
    P78324
  • Protein/Peptide Name:
    SIRP alpha
  • Purity:
    >95% as determined by SDS-PAGE.
  • Molecular Weight:
    63.5 kDa
  • Endotoxin Level:
    Low
  • Formulation:
    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
  • Shipping Temperature:
    Powder,RT

Specifications